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Flow cytometry facilities

As the original high content analysis technique, flow cytometry is an essential tool for cellular analysis.

A flow cytometer has the ability to make multiple measurements on individual cells and to do that on thousands of cells per second, which can reveal the heterogeneity in cell populations hidden by other techniques.

On flow cytometers with the “sorting” capability, it is possible to go beyond analysis of the sample and physically separate out particular cell populations for further analysis by other techniques, or to put them in to culture.

Equipment

  • Flow cytometry
    • 2 x BD LSR Fortessa
    • BD LSR-II
    • BD FACSCanto-II
    • BD FACSVerse
    • BD Influx
    • BD FACSAria Fusion
  • Imaging cytometry
    • Amnis ImageStreamX
  • Mass cytometry
    • Helios-CyTOF
  • Data analysis
    • Flowjo
    • Cytobank
    • R packages

Services

  • A number of flow cytometers are available for analysing fluorescently labelled samples with configurations capable of detecting up to 17-colour in a single sample
  • Cell sorting up to 16-colours and 6 populations simultaneously into an array of tubes, plates and slides
  • Imaging and analysis of labelled cells with up to 9-fluorescent labels
  • Analysis of up to 40 antibodies in a single sample using mass cytometry

Contact

Dr Gareth Howell, Facility Manager
E: gareth.howell@manchester.ac.uk
T: +44 (0)161 275 1742

Mr Mike Jackson, Senior Technician
E: m.c.jackson@manchester.ac.uk
T: +44 (0)161 275 5468

Futher information

More information can be found at the following links:

Flow Cytometry Facility

Mass Cytometry