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DNA sequencing facilities

The DNA Sequencing Facility provides high quality, high throughput sequence data to the entire campus of the University of Manchester and beyond.

The facility operates two primary platforms; one for Sanger sequencing and one for metagenomic analyses and related next-generation sequencing projects. The facility additionally offers human cell line authentication and mycoplasma testing of cell lines.

The sequencing facility processes thousands of samples per week, contributing data to hundreds of projects both within the Faculty and further afield.

Both Sanger sequencing and Roche 454 pyrosequencing can provide read lengths of >1000bp. Sequencing facility staff are on hand to provide troubleshooting, sequencing advice, and assistance with sequence data analysis.

Equipment

  • ABI 3730 DNA analyser
  • Roche 454 GS Junior+
  • Agilent 2100 Bioanalyzer
  • Beckman Coulter Biomek 3000
  • Eppendorf Epmotion 5075
  • NanoDrop ND-1000
  • Qubit 2.0 Fluorometer
  • ABI Genemapper software

Services

The sequencing facility offers two levels of Sanger sequencing; pre-mixed samples and load-only samples.

Pre-mixes are a mix of primer and template to be sequenced and processed at the facility, whilst load-only samples have been pre-sequenced, precipitated to a dry pellet and delivered to the facility to be loaded onto the sequencer.

The facility also offers genotyping services (eg microsatellite, TRFLP analysis), human cell line authentication (STR analysis) and PCR-based mycoplasma testing.

Long-read next-generation sequencing can typically provide >150 000 reads in a single run. Read lengths of up to 1100bp in a single read (not paired-end) are readily achievable.

The Roche 454 GS Junior has proven to be ideal for metagenomics projects, which benefit from this long read length providing enhanced coverage of the gene of interest.

Please contact the facility directly for information on sample preparation and delivery.

Contacts

  • Paul Fullwood
  • Fraser Combe

Email: dnasequencing@manchester.ac.uk

Tel: +44 (0)161 275 4867