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Biomolecular analysis facilities

The Biomolecular Analysis Core Facility is a state-of-the-art resource for the size, shape, stability and interactions of molecules.

The facility contains multiple biophysical analysis systems for the analysis of macromolecular interactions including surface plasmon resonance, biolayer interferometry, calorimetry and microscale thermophoresis.

It is also innovative in the use of biophysics to study surface layers such as glycosaminoglycans and lipid bilayers, as well as investigating cell interactions and mechanobiology using imaging interferometry, multi-parametric SPR and QCM-D.

The facility has expertise in analytical ultracentrifugation and dynamic/classical light scattering techniques for the analysis of molecular size and shape, aggregation and oligomerisation.

It also runs a protein quality control service which includes light scattering and thermofluor stability analysis as well as a buffer 48-condition buffer screen.

All work is supported by high resolution electrophoretic and chromatographic micro separation systems.

There are three dedicated facility staff who provide service and support for the most popular applications as well expertise in developing and establishing new methodologies using the equipment.

Equipment

  • Analytical ultracentrifugation (XLI and XLA)
  • Isothermal titration calorimetry (Malvern PEAQ)
  • Circular dichroism (Jasco J810 CD)
  • Fluorescence spectroscopy (Horiba scientific)
  • Multi-angle light scattering (Wyatt 18-angle)
  • Dynamic light scattering (Malvern Nano-S)
  • Uncle thermofluor/DLS (Unchained Labs)
  • Surface plasmon resonance (Biacore T200)
  • Biolayer interferometry (Octed Red 96)
  • Quartz Crystal Microbalance (Q-sense Omega)
  • FPLC (Automated Bio-Rad NGC)
  • Microscale thermophoresis (Nanotemper Pico)
  • Contrast enhanced imaging with interferometry (N-Lab)
  • MP-SPR automated (Bionavis 220)
  • MP-SPR for live cells (Bionavis 400)

Services

The facility has been set up to promote and facilitate the use of biomolecular analysis techniques.

There are a number of different modes of interaction with the facility including:

  • tailored project advice;
  • full service provision;
  • help with grant applications;
  • project work involving facility staff, and:
  • supported instrument access.

Contact

Dr Tom Jowitt, Biomolecular Analysis
Email: t.jowitt@manchester.ac.uk
Tel: +44 (0)161 306 5176